- Biomolecular Interaction Analyzer
- Automatic Pipetting Workstation
- Single Dispenser
GELICHEMᵀᴹ SPR Auto Biomolecular Interaction Analyzer
GELICHEMᵀᴹ SPR Auto is a desktop molecular interaction analyzer based on MetaSPR biosensors that analyzes interactions between two or more biomolecules in real-time without labeling, providing high-quality biological information on kinetics, affinity, specificity and concentration.
◈ High-precision liquid circuit control system with new fluid drive method for volumetric and syringe pumps, which substantially improves the stability of the liquid circuit and reduces the signal noise of data detection.
◈ Precise injection control with an integrated injection valve equipped with a high-precision dosing ring and a high-precision syringe pump for precise automated injection and consistent sample volume.
◈ The testing area is equipped with a constant temperature control system in the range of 4-40℃, with a temperature control accuracy of ±0.5℃, ensuring the consistency of the ambient temperature throughout the experimental process.
◈ Intuitive, convenient and powerful data acquisition and control software that is easy to use.
Disposable dual-channel microfluidic chip
Application Cases
Determination of the affinity of antibody drugs to target proteins
◈ Test chip: anti-human Fc (AHC) biochip;
◈ Immobilised protein: Herceptin monoclonal antibody;
◈ Analyte: HER2 protein.
The KD values are calculated in two system, the results are coincident.
SA Biochip affinity assay
◈ Test chip: SA Biochip;
◈ Immobilised protein: Biotin-ACE2;
◈ Analyte: SARS-CoV-2 RBD protein.
The KD values are calculated in two system, the results are coincident.
Tech Specs
Detection Principle | MetaSPR technology |
Injection Volume | 50-500μL |
Temperature | 4-40℃ |
Buffer Selection | switching between two buffers |
Automatization | Automatic |
Flow Rate | 5-600μL/min |
Capacity | 1*96-well plate |
Export File | EXCEL，TXT，JPG |
Concentration Range | Sample concentration > 1 pM |
Analysis Time per Cycle | 5 to 15min |
Association Rate Constant (ka) | Proteins : 101 - 107 M-1s-1 LMW molecules：103 - 5×107 M-1s-1 |
Minimum Molecular Weight for Molecular Interaction Analysis | 150 Da |
Dissociation Rate Constant (kd) | 10-6-10-1 s-1 |
Affinity Constant（KD） | pM-mM |
Baseline Drift | < 0.2RU/min |
Baseline Noise (RMS) | 0.1RU(RMS) |
Sample Processing | Auto Sampling Mechanism analyze huge sample for a long time，24h unattended operation |
Dimensions（H x W x D） | 697*500*470 mm |
Weight | 60kg |
Applications
Kinetics/affinity characterization, kinetics/affinity screening, single-cycle kinetics, LMW interaction analysis, fragment screening, epitope mapping1, immunogenicity, concentration analysis, calibration-free concentration analysis.
Data Presentation
Graphs displaying real-time kinetic binding sensorgrams, fitted result plots,concentration data analysis including calibration curves and output of tabulated concentration data, tabulated kinetic data, epitope identification plots and bar charts.